Journal: Journal of Biological Engineering

Article Title: Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow

doi: 10.1186/s13036-025-00491-2

Figure Lengend Snippet: Human ASC osteogenesis is supported by culture on SBG-PLGA composites and rhBMP-2 treatment. mRNA levels of the early and late osteoblastic markers, BMP-2 and Noggin ( NOG ) in human ASCs cultured on SBG-PLGA composites in ( A ), ( C ) standard osteogenic medium or ( B ), ( D ) standard osteogenic medium supplemented with 100 ng/ml rhBMP-2. Results are presented as relative mRNA expression levels vs. mRNA levels for ASCs cultured on a plain PLGA control (black line at 1). ( E ) Nitric oxide (NO) concentration in culture media after 24-h culture of ASC cells on SBG-PLGA composites in standard osteogenic medium. Averages ± SD are indicated. One-way or two-way ANOVA tests, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the PLGA control group

Article Snippet: ASC52telo cells (ASC; ATCC, SCRC-4000) and normal human ASCs (ATCC, PCS-500-011) were expanded in the dedicated medium (ATCC, Mesenchymal Stem Cell Basal Medium with Mesenchymal Stem Cell Growth Kit and G418).

Techniques: Cell Culture, Expressing, Control, Concentration Assay

Journal: Journal of Biological Engineering

Article Title: Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow

doi: 10.1186/s13036-025-00491-2

Figure Lengend Snippet: Cumulative osteogenic effect of Phenamil and PD98059 treatment in rhBMP-2 stimulated human ASCs cultured on SBG-PLGA composites. mRNA levels of osteoblastic markers in ( A ) 7-day and ( B ) 21-day ASC cultures on SBG-PLGA composites. ASCs were cultured in osteogenic medium supplemented with 100 ng/ml rhBMP-2 or 100 ng/ml rhBMP-2, 50 µM PD98059 and 20 µM Phenamil. Results are presented as relative mRNA expression compared to mRNA levels in control cells cultured on PLGA with rhBMP-2 only (marked as black line at 1). ( C ) mRNA levels of selected osteoblastic markers in 3-day osteogenic ASC cultures treated with different doses of rhBMP-2 (25–250 ng/ml), Phenamil (5–50 µM) or PD98059 (1-125 µM); under fluid shear stress. Results are presented as the expression relative to osteogenic cultures treated only with ascorbic acid, dexamethasone and β-glycerophosphate. ( D ) Graphical hypothesis of BMP-2, PD98059 and Phenamil cross-talk in intracellular signaling. Average values ± SD are indicated. Two-way ANOVA test, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the PLGA control group or between marked groups. BMP-2 – bone morphogenetic protein 2, OC – osteocalcin, ON – osteonectin, FOS – AP-1 transcription factor subunit (c-fos), OPG – osteoprotegerin

Article Snippet: ASC52telo cells (ASC; ATCC, SCRC-4000) and normal human ASCs (ATCC, PCS-500-011) were expanded in the dedicated medium (ATCC, Mesenchymal Stem Cell Basal Medium with Mesenchymal Stem Cell Growth Kit and G418).

Techniques: Cell Culture, Expressing, Control, Shear

Journal: Journal of Biological Engineering

Article Title: Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow

doi: 10.1186/s13036-025-00491-2

Figure Lengend Snippet: Fluid shear stress strengthens the osteogenic effects of rhBMP-2, PD98059 and Phenamil in ASCs cultured on SBG-PLGA composites. mRNA levels of osteoblastic markers after 7-day ASC culture on SBG-PLGA composites in ( A ) standard osteogenic medium under either static conditions or with fluid shear stress; and ( C ) osteogenic medium supplemented with 100 ng/ml rhBMP-2, 50 µM PD98059 and 20 µM Phenamil under either static conditions or with fluid shear stress. Results are presented as relative mRNA expression levels compared to mRNA levels in a control, static culture on PLGA (marked as a black line at 1). ( B ) The method of fluid shear stress application in ASC cultures using a standard laboratory see-saw rocker (7° tilt angle, 6 RPM frequency). ( D ) F-actin distribution in ASCs (Phalloidin-Atto488, magenta colored) at day 3 of culture in osteogenic medium supplemented with rhBMP-2, PD98059 and Phenamil after continuous static or dynamic culture conditions applied for 3 days. Scale bar represents 100 μm. ( E ) Western blot (WB) analysis of p-ERK1/2 and p-SMAD1/5/8 in ASCs after 1-h treatment with rhBMP-2 or rhBMP-2, PD98059 and Phenamil in static or dynamic conditions (upper panel) along with densitometric quantifications of WB results normalized to GAPDH levels. Averages ± SD are indicated. Two-way ANOVA test, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the static PLGA control or between marked groups

Article Snippet: ASC52telo cells (ASC; ATCC, SCRC-4000) and normal human ASCs (ATCC, PCS-500-011) were expanded in the dedicated medium (ATCC, Mesenchymal Stem Cell Basal Medium with Mesenchymal Stem Cell Growth Kit and G418).

Techniques: Shear, Cell Culture, Expressing, Control, Western Blot

Journal: Journal of Biological Engineering

Article Title: Rapid osteoinduction of human adipose-derived stem cells grown on bioactive surfaces and stimulated by chemically modified media flow

doi: 10.1186/s13036-025-00491-2

Figure Lengend Snippet: Zinc (ZnO) or strontium (SrO) modified SBG-PLGA composites combined with fluid shear stress and BMP-based chemical stimulation, further increase osteogenic markers expression in early ASC cultures. mRNA levels of osteoblastic markers in ( A ) 3-day and ( B ) 6-day osteogenic ASC cultures on PLGA-based composites containing either unmodified or SrO- or ZnO-modified SBGs. Cells were treated with a combination of rhBMP-2, PD98059 and Phenamil at the indicated culture times in either static cultures or under fluid shear stress. Upper panels show the schemes of the ASC treatments. Results are presented as relative mRNA expression levels vs. mRNA levels in a control, static culture on PLGA (marked as a black line at 1). ( C ) Western blot (WB) analysis of phospho-β-catenin(Ser552), COX-2 and phospho-CREB(Ser133) levels in ASCs after 1-h treatment with rhBMP-2 or rhBMP-2, PD98059 and Phenamil in static or dynamic conditions (left panel) along with densitometric quantifications of WB results normalized to GAPDH levels (right panel). ( D ) Hypothesized signaling pathways involved in osteogenic response to treatment strategy. Averages ± SD are indicated. Two-way ANOVA test, * p < 0.05, ** p < 0.001, *** p < 0.0001 relative to the respective static PLGA control group or between marked groups

Article Snippet: ASC52telo cells (ASC; ATCC, SCRC-4000) and normal human ASCs (ATCC, PCS-500-011) were expanded in the dedicated medium (ATCC, Mesenchymal Stem Cell Basal Medium with Mesenchymal Stem Cell Growth Kit and G418).

Techniques: Modification, Shear, Expressing, Control, Western Blot, Protein-Protein interactions

Journal: Journal of Personalized Medicine

Article Title: The Effect of Ionizing Irradiation on the Autotaxin-Lysophasphatidic Acid Axis and Interleukin-6/8 Secretion in Different Breast Cancer Cell Lines

doi: 10.3390/jpm14090968

Figure Lengend Snippet: Alterations in ATX expression on the mRNA level of ASC/TERT1 cells 24 h after stimulation with IL-6 ( A ) and IL-8 ( B ). The columns show the mean relative ATX expression (y-axis) in varying concentrations of IL-6 and IL-8 (x-axis) compared to YWHAZ and to the control group. Values were calculated using the 2 −ΔΔCT method, where 2 −ΔΔCT of control = 1. * p < 0.05 (Mann–Whitney U test).

Article Snippet: An ASC/TERT1 (human-adipose-tissue-derived telomerase-immortalized mesenchymal stem cell line) was purchased from Evercyte (Evercyte GmbH, Vienna, Austria) and cultivated in Endothelial Cell Growth Medium (EGM)-2 BulletKit (Lonza Group AG, Basel, Switzerland).

Techniques: Expressing, Control, MANN-WHITNEY